| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | JOHNSON, Jaquavia | SO | Jefferson | 27.89 |
| 2 | PATTERSON, Kyra | JR | George Steinbrenner | 26.40 |
| 3 | MARTINEZ, Vanessa | SR | Tampa Bay Tech | 26.39 |
| 4 | HOLMES, Makayla | JR | Plant City | 26.30 |
| 5 | SESSIONS, Myllanie | JR | King | 26.31 |
| 6 | BRANCHE, Isabelle | SO | Newsome | 26.40 |
| 7 | BALDWIN, Jada | SO | Plant | 26.94 |
| 8 | DURAN, Angelise | SO | George Steinbrenner | 28.05 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | MILLER, Kaelyn | JR | Wharton | 26.17 |
| 2 | HOLLOMAN, Erica | SR | Plant City | 25.70 |
| 3 | STERLING, Oivia | JR | Armwood | 25.42 |
| 4 | BRAZELL, Serenity | SR | Wharton | 24.85 |
| 5 | SNOW, Zekira | SR | Bloomingdale | 25.10 |
| 6 | ROBERSON, Ja'Mya | JR | Hillsborough | 25.68 |
| 7 | RANDELL, Nia | SR | Hillsborough | 25.82 |
| 8 | SAMPSON, Amirah | FR | Lennard | 26.30 |