| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Robinson |
A | Milsaps | White | Taggart | Tate | 10:19.28 |
| 2 | Hillsborough |
A | Reed | Randell | Pulizzano | Burke | 10:35.22 |
| 3 | Tampa Bay Tech |
A | Antoine | Brown | Purdy | Wright | 10:40.48 |
| 4 | Sickles |
A | Blackford | Swanton | Raquet | Kontz | 10:52.35 |
| 5 | Wesley Chapel |
A | Gattuso | Boateng | Salomon | Michel | 10:53.97 |
| 6 | Freedom (Tampa) |
A | Ammirati | Komara | Le | Selvaggi | 11:04.52 |
| 7 | Bishop McLaughlin |
A | Byrne | Crosswhite | Deehan | Donis | 11:22.97 |
| 8 | Springstead |
A | Jimenez Capo | Brewer | Lindsey | Rosario | 11:30.81 |
| 9 | Armwood |
A | Scott | Albert | Lowder | Estivene | NT |
| 10 | Alonso |
A | Richardson | Jones | Howell | Martinez | NT |
| 11 | Wiregrass Ranch |
A | Schmitt | Gaona-Zelaya | Johnson | Burkett | NT |