| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BAILEY, Abigail | Tampa Bay HEAT |
NT |
| 2 | PASTRANA, Xamara | Springstead |
1:38.04 |
| 3 | COX, Jayla | Wharton |
1:23.81 |
| 4 | STOLTZFOOS, Scotlyn | Gaither |
1:22.63 |
| 5 | LEWIS, Annabel | Wesley Chapel |
1:23.71 |
| 6 | EDWARDS, Jordann | Armwood |
1:27.22 |
| 7 | CLARKE, Khloe | Central (Brooksville) |
1:45.94 |
| 8 | ZAPATA, Gianna | Lecanto |
NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CLEAR, Chelsea | Wiregrass Ranch |
1:20.76 |
| 2 | JOHNSTON, Madissen | Alonso |
1:19.80 |
| 3 | BURKARDT, Gabriella | Wesley Chapel |
1:18.13 |
| 4 | ATIENZA, Ramya | Winthrop College Prep | 1:16.90 |
| 5 | MUKUSHEVA, Nesibelli | Alonso |
1:17.50 |
| 6 | CHAMBERLAIN, Olga | Alonso |
1:19.64 |
| 7 | FARMER, Emery | Cypress Creek (Wesley Chapel) |
1:20.33 |
| 8 | JANKOVIC, Maya | Bishop McLaughlin |
1:22.38 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | RODRIGUEZ, Shannell | Tampa Bay Tech |
1:14.40 |
| 2 | POLITE, Ca'Miyah | Hillsborough |
1:11.78 |
| 3 | DENNING, Nandi | Wesley Chapel |
1:06.90 |
| 4 | MILLER, Adrianna | Lecanto |
1:05.81 |
| 5 | LOVETT, Karinah | Hillsborough |
1:06.19 |
| 6 | CINEAS, Ayanna | Tampa Bay Tech |
1:07.07 |
| 7 | SAYRE, Viktoria | Springstead |
1:13.41 |
| 8 | CONRAD, Sydney | Wiregrass Ranch |
1:15.52 |