| Lane | # | Athlete | Age | Team | Seed |
|---|---|---|---|---|---|
| 2 | 469 | YOUNG-MOORE, Chlo'e | 5 | ENDURER'S 4 Christ | NT |
| 3 | 490 | STALLINGS, Isabella | 5 | NORTH FLORIDA EXPRESS | NT |
| 4 | 533 | MEDLEY, De'Nauri | 4 | V12 Athletics | NT |
| 5 | 466 | PERRY, Kennedy | 5 | ENDURER'S 4 Christ | NT |
| 6 | 420 | BROWN, Mariyah | 5 | Athletics Academy Elite | NT |