| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 1 | NUNEZ, Eva | Plant | NT | 
| 2 | LEWIS, Margaret | Plant | NT | 
| 3 | CHEESMAN, Carson | Plant | NT | 
| 4 | GLOR, Kassandra | Blake | 4:15.98 | 
| 5 | UBAU, Penelope | Blake | 5:00.00 | 
| 6 | FERRER, Mary | Plant | NT | 
| 7 | CONNERY, Ava | Plant | NT | 
| 8 | GAGNIER, Helena | Robinson | 2:45.73 | 
| 9 | GEIGER, Emily | Plant | NT | 
| 10 | GARUTTO, Ella | Robinson | NT | 
| 11 | HOLLYFIELD, Alessandra | Robinson | NT | 
| 12 | RAMOS, Serena | Blake | 3:44.43 | 
| 13 | MILLER, Madison | Plant | NT |