| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Chasco K8 | A | Stephens | Yavorshi | John | Anderson | 1:02.68 |
| 4 | Starkey Ranch K-8 | A | Paradayil | Bossone | Julian | Barr | 59.58 |
| 5 | Paul R. Smith Middle School | A | Golden | Ford | Sephus | Castor | 1:01.97 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Bayonet Point Patriots | A | Moran | Smith | Badger | Starks | 58.09 |
| 3 | Crews Lake Middle School | A | Butler | Jackson | Owens-Hines | Stoltz | 56.90 |
| 4 | Hudson Middle School | A | Johnson | Klinger | Streitenberger | Lee | 55.10 |
| 5 | Seven Springs Middle School | A | Otto | Michalski | Owens | Byrd | 56.42 |
| 6 | River Ridge Knights | A | Evans | Lovell | Ramos | Girardi | 57.40 |
| 7 | Gulf Middle School Buccaneers | A | Lewis | Solis | Arnold | Hall | 59.33 |