| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Chasco K8 | A | Anderson | Yavorshi | John | Stephens | 1:03.20 |
| 3 | Starkey Ranch K-8 | B | Shea | Heck | Kaster-Sadler | Hach | 59.62 |
| 4 | River Ridge Knights | A | Weaver | Bendall | Papapanos | Girardi | 57.40 |
| 5 | Gulf Middle School Buccaneers | A | Louiseau | Solis | Arnold | Hall | 59.34 |
| 6 | Starkey Ranch K-8 | A | Paradayil | Bossone | Julian | Barr | 59.62 |