| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BALSER, Madison | Chasco K8 | 3:08.00 |
| 2 | BRADSHAW, Alina | Starkey Ranch K-8 | NT |
| 3 | DOBBINS, Lily | Starkey Ranch K-8 | NT |
| 5 | DURRETT, Kaliyah | Seven Springs Middle School | NT |
| 6 | HENCZ, Brooklyn | Seven Springs Middle School | NT |
| 7 | HOLMES, Gabriella | Gulf Middle School Buccaneers | NT |
| 8 | KATSIMBRAKIS, Alexandria | Starkey Ranch K-8 | NT |
| 9 | LAWSON, Zoey | Seven Springs Middle School | NT |
| 10 | MURPHY, Maura | Starkey Ranch K-8 | NT |
| 11 | SOLANO-VALDEZ, Perla | Chasco K8 | NT |