| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ROUNDTREE, Demarion | Big Lake Track | NT |
| 2 | SPEED, Marquise | Largo Elite Track Club | NT |
| 3 | REID, William | Olympia Track Club | NT |
| 4 | JONES, Braylon | Big Lake Track | NT |
| 5 | HOERNLEIN, Sean | Olympia Track Club | NT |
| 6 | BRADFORD JR., Javon | Largo Elite Track Club | NT |
| 7 | BRUNDAGE, Braylene | Largo Elite Track Club | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | FRAZER, Andre | AsylumMade | 26.26 |
| 2 | BRICENO, Nikolai | AsylumMade | 25.94 |
| 3 | KIRWAN, Johnathan | 813 Elite Track and Field | 25.17 |
| 4 | SMITH, Obisisa | Ballistic Track and Field | 23.50 |
| 5 | PETERSON, Mason | SPARQ Track Club | 25.06 |
| 6 | BROOKS, Kaden | Manatee Mustangs SportsAcademy | 25.79 |
| 7 | REID, Romello | AsylumMade | 26.24 |
| 8 | DAVIS, Brian | Manatee Mustangs SportsAcademy | 28.80 |