| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GULLEY, Markese | Largo Elite Track Club | NT |
| 2 | OLIVER, Thaddeus | Big Lake Track | NT |
| 3 | PIÑA JR, Erick | AsylumMade | 31.21 |
| 4 | JONES, Silas | Track life Sports | 30.00 |
| 5 | JACKSON, Markus | SPARQ Track Club | 30.97 |
| 6 | BRADBERRY, Jayden | 813 Elite Track and Field | NT |
| 7 | GOFF, Kyle | SPARQ Track Club | NT |
| 8 | JUSTICE, Micah | 813 Elite Track and Field | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GLAUDE, Amari | 813 Elite Track and Field | 28.66 |
| 2 | SCHUMMER, Graham | Unattached | 28.04 |
| 3 | GRAY, King | AsylumMade | 27.32 |
| 4 | MOREHEAD, Ashton | Westchase Cheetahs Track Club | 26.93 |
| 5 | MULLER, Dae'Lyn | SPARQ Track Club | 27.29 |
| 6 | LEE, Jaxson | Olympia Track Club | 28.00 |
| 7 | HAWK, Connor | Ohio Speed Demons | 28.60 |
| 8 | LEVY, Ryleigh | AsylumMade | 29.49 |