| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | FRANKLIN, Sarai | Ballistic Track and Field | NT |
| 3 | NEAL, Avari | Olympia Track Club | NT |
| 4 | KENNEDY, Amiya | My Brothers & Sisters Keeper | 39.90 |
| 5 | KENNEDY, Amani | My Brothers & Sisters Keeper | 41.20 |
| 6 | KING, Reagan | Big Lake Track | NT |
| 7 | MICHEL, Keilani | Olympia Track Club | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CRUZ, Seleta | 813 Elite Track and Field | 32.90 |
| 2 | SAWYER-HILL, Waylen | Weston Warriors | 31.34 |
| 3 | PACKER, Amora | Unattached | 31.00 |
| 4 | REED, Elayna | Westchase Cheetahs Track Club | 29.91 |
| 5 | KEARSE, Tearia | Olympia Track Club | 30.94 |
| 6 | KENDRICKS, Malaya | Burg Blazers Athletics | 31.24 |
| 7 | RANDALL, Aziah | AsylumMade | 31.70 |
| 8 | MEREUS, Addison | My Brothers & Sisters Keeper | 34.80 |