| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | KING, De'Asia | Big Lake Track | NT |
| 2 | SMITH, Diari | Ballistic Track and Field | NT |
| 3 | JOHNSTON, Harmony | 813 Elite Track and Field | NT |
| 4 | HILL, Niloni | Big Lake Track | NT |
| 5 | BAILEY, Reign | Olympia Track Club | NT |
| 6 | PATTERSON, D'Asia | Big Lake Track | NT |
| 7 | EARLEY, Kristen | Largo Elite Track Club | NT |
| 8 | FORTUNE, Calia | Big Lake Track | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GILLIAM, Dreya | SPARQ Track Club | NT |
| 2 | REDDICK, Zoe | Largo Elite Track Club | NT |
| 3 | SPEIGHTS, Jaoni | Ballistic Track and Field | 45.97 |
| 4 | COLEMAN, Armanii | Burg Blazers Athletics | 38.67 |
| 5 | SMITH, Madyson | SPARQ Track Club | 39.50 |
| 6 | YOUNG, Seriyah | Olympia Track Club | NT |
| 7 | MICHEL, Keilani | Olympia Track Club | NT |
| 8 | SUMPTER, Amia | Big Lake Track | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GREENE, Amara | Ballistic Track and Field | 37.99 |
| 2 | MURPHY, Aubri | Ballistic Track and Field | 37.34 |
| 3 | DORSEY, Shaviya | Largo Elite Track Club | 36.33 |
| 4 | JENKINS, Jayla | Ballistic Track and Field | 34.60 |
| 5 | REED, Elexis | Westchase Cheetahs Track Club | 35.52 |
| 6 | MILLER, Imani | Ballistic Track and Field | 37.06 |
| 7 | JELKS, Dominese | Ballistic Track and Field | 37.53 |
| 8 | LOVETT, Chasity | 813 Elite Track and Field | 38.57 |