| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | HIERS, Karter | Largo Elite Track Club | NT |
| 3 | PAYNE, Jayvion | Olympia Track Club | NT |
| 4 | SMITH, Israel | Ballistic Track and Field | NT |
| 5 | COSTON, Artavian | Ballistic Track and Field | NT |
| 6 | SHANNON III, Billy | Big Lake Track | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | POWELL, Timarus | AP6 Absolute Pressure | NT |
| 3 | DAVIS, Kiyon | AsylumMade | 49.42 |
| 4 | ABNER JR, Marcus | SPARQ Track Club | 35.27 |
| 5 | SALLYE, Kameron | My Brothers & Sisters Keeper | 44.30 |
| 6 | LASSITER, Tristan | Ballistic Track and Field | NT |