| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | WEST, Sahrai | Olympia Track Club | NT |
| 3 | REED, Amia | My Brothers & Sisters Keeper | 55.24 |
| 4 | FARRELL, Leyiani | Ballistic Track and Field | 48.70 |
| 5 | JONES, Alauna | 813 Elite Track and Field | 52.17 |
| 6 | JOHNSON, Kennedy | Largo Elite Track Club | NT |
| 7 | LIVE-BOYD, Jimmeria | Manatee Mustangs SportsAcademy | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CRUZ, Sevyn | 813 Elite Track and Field | 45.05 |
| 2 | WALKER, Amor | Ballistic Track and Field | 44.39 |
| 3 | RICHARDSON, Laila | Burg Blazers Athletics | 41.74 |
| 4 | CARTER, Ava | Olympia Track Club | 35.84 |
| 5 | SAWYER-HILL, Landen | Weston Warriors | 41.24 |
| 6 | GERMAN, Sol | 813 Elite Track and Field | 43.52 |
| 7 | GAMBLE, Amaya | 813 Elite Track and Field | 45.00 |
| 8 | ATKINS, Beatrix | SPARQ Track Club | 48.45 |