| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Largo Elite Track Club | B | Brundage | Gardiner | Jones | Swain | DNS |
| 3 | SPARQ Track Club | A | Hannon | Peterson | Ogden | Porter | NT |
| 4 | AsylumMade | A | Frazer | Randall Jr | Levy | Briceno | 52.29 |
| 5 | Largo Elite Track Club | A | Emery | Bradford Jr. | Gulley Jr | Speed | 53.00 |