| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Morgan High School | A | Polanco | Houston | Moore | Bois | 46.19 |
| 4 | Middleton |
A | Hardy | Stanford | Prassanna | Smith | 45.21 |
| 5 | Brandon |
A | Byrd | Charles | Nelson | Gray | 45.44 |
| 6 | Leto |
A | Blankenship | Adams | Cruz | White | 47.82 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Jefferson |
A | Dubose | Washington | Holmes | Bowens Jr | 45.08 |
| 2 | Freedom (Tampa) |
A | Beaubrun | Lambert | Dye | Bernard | 44.65 |
| 3 | East Bay |
A | Alexander | Koonce | Person | McClary | 43.88 |
| 4 | Blake |
A | Newman-Hall | Lucas | Lormera | Shaw | 43.60 |
| 5 | King |
A | Levine | Sawaengsri | Lefevre | Theus | 43.84 |
| 6 | Riverview |
A | Bailey | Tatum-Lesane | Lugo | Holliday | 44.58 |
| 7 | Sickles |
A | Fernandez | Kieffer | Sandos | Thompson | 44.78 |
| 8 | George Steinbrenner |
A | Arevalo | Hughes | Murphy | Wyllys | 45.18 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Strawberry Crest |
A | Weems | von Ancken | Nguyen | Brown | 43.36 |
| 2 | Spoto |
A | Jones | Brown | Berry | Bernard | 43.05 |
| 3 | Bloomingdale |
A | Burton | Lowe | Smith | Frank | 42.81 |
| 4 | Hillsborough |
A | Doe | Haynes | Garcia | McMillian | NT |
| 5 | Chamberlain |
A | Viera Lozano | DeLoatch | Ford | Winns | 42.55 |
| 6 | Plant |
A | Bennett | Daniels | Williams | Latherow | 42.96 |
| 7 | Newsome |
A | Metelus | Haggans | Jones | Holt | 43.24 |
| 8 | Alonso |
A | Galley | Albury | Alexander | Casanova | 43.52 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Sumner |
A | Taylor | Jones | Haugabook | Scott | 42.11 |
| 2 | Durant |
A | Kenne | Porter | Hoddnett | Jefferson | 41.86 |
| 3 | Plant City |
A | Hugee | Copeland | Urena | Woodley | 41.67 |
| 4 | Tampa Bay Tech |
A | Zayas | Gray | Floyd | Daniel | 41.09 |
| 5 | Armwood |
A | Adams | Clark | Jones | Hudgins | 41.22 |
| 6 | Gaither |
A | Balloon | Kindred | Hamilton | Hamilton | 41.80 |
| 7 | Wharton |
A | Nesmith | Hilton | Walker | Stratford | 41.92 |
| 8 | Lennard |
A | Pestano | Ivery | Edwards | Green | 42.16 |