| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Wharton |
A | Jones | Mireles Alcantara | James | Canady | NT |
| 3 | Chamberlain |
A | NT | ||||
| 4 | Middleton |
A | Johnson | Carter | Ford | Pena | NT |
| 5 | Newsome |
A | Browne | Dawson | Ruddock | Crawford | NT |
| 6 | Chamberlain |
A | Nash | Nash | Nash | Nash | NT |
| 7 | Freedom (Tampa) |
A | Kolar | Lawson | Edwards-Ricketts | Wood | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Durant |
A | Bland | Howard | Monzon-Hidaldo | Darrow | NT |
| 2 | Alonso |
A | Galan | Lauricella | Ritzenthaler | Sanchez | 2:30.08 |
| 3 | Leto |
A | Allen Chona | Singh | Singh | Vargas Figueroa | 2:15.67 |
| 4 | Bloomingdale |
A | Exil | Spear-Zuleta | Ground | Sanchez | 2:04.05 |
| 5 | Strawberry Crest |
A | Georges | Perez | Carr | Santvil | 2:13.32 |
| 6 | Jefferson |
A | Brito | Bradford | Nunn | Grubbs | 2:16.67 |
| 7 | Morgan High School | A | Payton | Henry | Carter | Huggins | NT |
| 8 | Riverview |
A | Garlic | Lee-Shung | Campbell | Holdip | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Spoto |
A | Lopez | Snead | Johnson | Cherident | 2:02.91 |
| 2 | Sumner |
A | Bridges | Todman | Barton | Accilien | 2:00.12 |
| 3 | East Bay |
A | Hall | Rice | Jackson | Sherman | 1:59.17 |
| 4 | Plant |
A | Martin | Bryce-Wells | Boothe | Foster | 1:56.40 |
| 5 | Tampa Bay Tech |
A | Ash | Garmon | Daise | Danzey | 1:58.28 |
| 6 | Armwood |
A | Ashley | Harper | Johnson | Harrison | 1:59.59 |
| 7 | Lennard |
A | Jorden | Callaway | Reynoso | Sampson | 2:02.74 |
| 8 | Hillsborough |
A | Eugene | Presley | Lovett | Reed | 2:03.88 |