| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Mitchell |
A | Connor | Dunning | Beach | Harrigin | 44.32 |
| 3 | River Ridge |
A | Biggs | Fuller | Scott | Velazquez | 43.68 |
| 4 | Gulf |
A | Brown | Carpenter | Coleman | Porter-Jackson | 42.92 |
| 5 | River Ridge |
B | Moore | Mercer | Hertog | Winstanley | 43.68 |
| 6 | Mitchell |
B | Alyea | Diehl | Moore | McClain | 44.32 |