| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Tampa Bay Tech |
A | Long | Hughes | Ferguson | Pugh | NT |
| 2 | Spoto |
A | Charles | Pointer | Harvey | Pierrelus | NT |
| 3 | Riverview |
A | Collazo | Sam | Thompson | Matthews | NT |
| 4 | Newsome |
A | Boone | Easley | Rubocki | Leifer | 1:17.39 |
| 5 | Bloomingdale |
A | Mills | Morman | Katsareas | DeVincenzo | 1:17.96 |
| 6 | Armwood |
A | Osley | Nereus | Hudgins | Desulme | NT |
| 7 | Middleton |
A | Camille | Repic | Johnson | Hardy | NT |
| 8 | George Steinbrenner |
A | Miller | South | Miller | Miller | NT |