| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Weeki Wachee |
B | Priester | Kernisant | Gilmore | Henderson | 53.86 |
| 3 | Hernando |
A | Delmont | Smith | McCook | Bennett | 52.89 |
| 4 | Weeki Wachee |
A | Benavente | Goodwin | Anderson | Baker | 53.86 |
| 5 | Pasco |
A | Serdynski | Routier | Rivera | Davis | 56.62 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Central (Brooksville) |
A | Austin | Bailey | Miodowski | Hunt | 51.18 |
| 2 | Armwood |
B | Bowers | Hawkins | Huggins | Newton | 49.49 |
| 3 | Armwood |
A | Carr | Johnson | Gardiner | Reed | 49.49 |
| 4 | Armwood |
C | Edwards | Dickens | Gunn | Segar | 49.49 |
| 5 | Land O'Lakes |
A | Davis | Oneal | Maita | Stephens | 50.70 |
| 6 | Nature Coast |
A | Norton | Schotborgh | Peters | Page | 51.18 |