| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | AMMIRATI, Madelyn | Freedom (Tampa) | 12:00.00 | |
| 2 | PULIZZANO, Hazelle | Hillsborough | 12:30.00 | |
| 3 | ST JOHN, Analyse | Lennard | 12:30.00 | |
| 4 | BRICKLEMYER, Edith | Hillsborough | 13:30.00 | |
| 5 | BURKE, Audrey | Hillsborough | 13:30.00 | |
| 6 | MEADOWS, Alicia | Lennard | 14:00.00 | |
| 7 | HICKS, Farah | Wharton | 15:30.00 | |
| 8 | BUIE, Keana | Wharton | 16:00.00 | |
| 9 | SAUER, Sophia | Wharton | 16:00.00 | |
| 10 | TORRES, Juliet | Lennard | 18:00.00 | |
| 11 | GOODISON, Victoria | Lennard | 19:00.00 | |
| 12 | NEUBERT, Amie | Lennard | 19:00.00 | |
| 13 | VAROUTAS, Madison | Lennard | 20:00.00 | |
| 14 | ALBERNAS, Isabella | Lennard | 22:00.00 | |
| 15 | CRUZ, Cristal | Lennard | 22:00.00 | |
| 16 | DIXON, Taylor | Wharton | NT | |
| 17 | CORBETT, Harper | Alonso | NT | |
| 18 | AZIE, Gabriella | Wharton | NT | |
| 19 | DOUGLAS, Sophia | Freedom (Tampa) | NT | |
| 20 | FREEMAN, Isabella | Alonso | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GONZALEZ, Emily | Lennard | NT | |
| 2 | HENRY, Kendall | Alonso | NT | |
| 3 | JONES, Alyza | Spoto | NT | |
| 4 | LEWIS, Ivey | Alonso | NT | |
| 5 | MARASCO, Caitlyn | Hillsborough | NT | |
| 6 | NGYUEN, Halley | Hillsborough | NT | |
| 7 | NOVELLO, Tesla | Freedom (Tampa) | NT | |
| 8 | OLMSTED, Dagny | Alonso | NT | |
| 9 | PICARD, Ella | Academy Lakes | NT | |
| 10 | RITZENTHALER, Vera | Alonso | NT | |
| 11 | SANCHEZ, Dilaila | Alonso | NT | |
| 12 | SCHUPAY, Sydney | Hillsborough | NT | |
| 13 | SHARMA, Tvisha | Alonso | NT | |
| 14 | SOTOMAYOR, Angela | Wharton | NT | |
| 15 | TRAN, Julianna | Alonso | NT | |
| 16 | VASQUEZ-CARDENAS, Melanie | Hillsborough | NT | |
| 17 | WATTS, Ellison | Academy Lakes | NT | |
| 18 | SETTY, Nora | Academy Lakes | NT | |
| 19 | WIMSATT, Lucille | Alonso | NT | |
| 20 | GEIB, Marley | Alonso | NT |