| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Honest Runners of North Miami | A | Charles | Perry | Canis | Darbeau-Gonzalez | NT |
| 4 | Boca Track Club | A | Johnson | Irons | Smtih | Poitier | 55.00 |
| 5 | Speed City Xpress Track Club | A | Dean | Williams | Leung-Smith | Bryan | NT |
| 6 | FC Razorbacks | A | Shannon | Shannon | Didier | Harris | NT |