| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hudson Academy | A | Peterson | White | Duessier | Streitenberger | 2:26.68 | Verified |
| 2 | Gulf Middle School | B | Arnold | Scott | Cook | Harris | 2:22.99 | Verified |
| 3 | River Ridge Middle School | A | 2:14.21 | Verified | ||||
| 4 | Starkey Ranch K-8 School | B | Axelsson | Cochol | Epstein | Dobbins | 2:11.75 | Verified |
| 5 | Starkey Ranch K-8 School | A | Leon | Scifo | Brady | LaBruzzo | 2:11.75 | Verified |
| 6 | Crews Lake Middle School | A | Butler | Jackson | Matticola | Stoltz | 2:19.63 | Verified |
| 7 | Gulf Middle School | A | Loiseau | Small | Slicker | Mexico | 2:22.99 | Verified |