| Alley | Hip | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|---|
| nullnull | undefined | Sarasota Military Academy |
A | Contreras | Guzman | Schlabach | Foss | 12:38.92 | ODA Thunder Classic 2025 |
| nullnull | undefined | Cardinal Mooney |
A | Fritschle | Horning | Maloni | Dib | 10:34.00 | North Port Invitational 2024 (Regional Qualifier) |
| nullnull | undefined | Riverview (Sarasota) |
A | Tenaglia | Hajkova | Logan | Los | 10:09.06 | Sarasota Riverview - Ram Invite 2025 |
| nullnull | undefined | Venice |
A | Arena | Maclean | Myburgh | Sieve | 10:01.60 | FHSAA 4A District 8 |