| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DONALDSON, Jamon | John Long Middle School | 25.88 | |
| 2 | MCFARLANE, Anquan | Hudson Academy | 25.56 | |
| 3 | PARNOFIELLO, Aydan | River Ridge Middle School | 25.17 | |
| 4 | CANTY, James | Pasco Middle School | 24.68 | |
| 5 | MILLER, Jakari | Bayonet Point Middle School | 24.71 | |
| 6 | DANGERFIELD, Emile | Pasco Middle School | 25.21 | |
| 7 | TOBIAS, William | Seven Springs Middle School | 25.77 | |
| 8 | CHARLES, Dominique | Cypress Creek Middle School | 25.98 |