| Meet Record | 48.36 | Alonso | 2013 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Plant | A | 53.03 | ||||
| 2 | George Steinbrenner | A | Duran | Hartley | Hernandez | Patterson | 52.57 |
| 3 | Riverview | A | Cesar | Rohm | West | Quiles | 51.78 |
| 4 | Armwood | A | 51.45 | ||||
| 5 | King | A | 51.49 | ||||
| 6 | Alonso | A | Morel | Kilkelly | Bartolo | Medina | 51.82 |
| 7 | Blake | A | Pringley | Smith | Lawson | Sims | 52.75 |
| 8 | Strawberry Crest | A | 54.04 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Sumner | A | Lovett | Harwell | Baroulette | Hyppolite | 51.36 |
| 2 | Wharton | A | Jackson | Gordon | Sutton | Dunlap | 49.99 |
| 3 | Tampa Bay Tech | A | Thomas | Miller | Gordon | Martinez | 49.15 |
| 4 | Hillsborough | A | Jones | ROBERSON | Smith | Murphy | 49.07 |
| 5 | Robinson | A | Williams | Rolack | Findlay | Jerson | 49.14 |
| 6 | Plant City | A | Holmes | Shaw | Holmes | Holloman | 49.96 |
| 7 | Lennard | A | Murrell | Luke | Peterson | Gray | 50.28 |
| 8 | Freedom (Tampa) | A | Anderson | Harrigan | Henderson | Kemp | DQ |