| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | THOMAS, A'Miah | Tri City Flyers | NT | |
| 4 | JONES, Tenia | Mississippi Trackstars | NT | |
| 5 | DENT, Briyell | St Charles Striders | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HUBBS, Irielle | Press Park Track Club | NT | |
| 2 | FRANKLIN, Asyria | ALL N TRACK | NT | |
| 3 | PELLEGRIN, Mollee | UDP Track Club | NT | |
| 4 | WILLIAMS, Fabrianna | Mississippi Trackstars | 31.65 | |
| 5 | WILLIAMS, Miquala | Trojan Elite Track | NT | |
| 6 | LUMPKINS, De'Arrion | ALL N TRACK | NT | |
| 7 | SAVAGE, Bennet | St john legends track club | NT | |
| 8 | WILSON, Izzy | Mississippi Trackstars | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MAIDEN, Aniyah | South Baton Rouge Jaguars Trac | 30.04 | |
| 2 | MATTHEWS, Adreyonna | Trojan Elite Track | 29.50 | |
| 3 | HUGHES, Kimora | U.S. Express Track Club | 28.98 | |
| 4 | OLIVERI, Alexandra | New Orleans Metro Stars Track | 28.00 | |
| 5 | ZANDERS, Asia | Trojan Elite Track | 28.90 | |
| 6 | BLACK, Natalie | Mississippi Trackstars | 29.05 | |
| 7 | RIGGINS, Paris | Press Park Track Club | 29.86 | |
| 8 | HARRIS, Braniyah | Press Park Track Club | 31.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HULBERT, C'Ira | Run Fast TC - Baton Rouge | 27.50 | |
| 2 | JONES, Nadia | Speed Dynamics | 27.25 | |
| 3 | LUCAS, Jaya | UDP Track Club | 26.83 | |
| 4 | MASON, Jalya | Run Fast TC - Baton Rouge | 24.78 | |
| 5 | ADEGBOYE, Joelle | Southeast Louisiana Roadrunner | 26.01 | |
| 6 | MCCOVINS, Da'Jroin | Run Fast TC - Baton Rouge | 27.00 | |
| 7 | NETTER, Jaleah | Run Fast TC - Baton Rouge | 27.50 | |
| 8 | COUSIN, Remi | New Orleans Metro Stars Track | 27.80 |