| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | ADDISON, Tyler | Unattached | NT | |
| 4 | OLIVER, Khole | Unattached | NT | |
| 5 | KENNEDY, Aubree | Tri City Flyers | NT | |
| 6 | GRACE, Padian | New Orleans Metro Stars Track | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SEYMORE, Krysten | U.S. Express Track Club | NT | |
| 2 | CRYER, Amanii | Trojan Elite Track | NT | |
| 3 | AKAMOHEARTEL, Arayah | U.S. Express Track Club | 34.71 | |
| 4 | LEWIS, Addison | U.S. Express Track Club | 31.30 | |
| 5 | CHARLES, Briley | UDP Track Club | 34.59 | |
| 6 | MADDING, Makenzye | Metropolitan Track Club | NT | |
| 7 | MIDDLETON, Arionna | Trojan Elite Track | NT | |
| 8 | JOHNSON, Persia | KY Track Club | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | STOKES, Saniah | UDP Track Club | 30.10 | |
| 2 | HARRIS, Briana | Westbank Track Stars | 28.00 | |
| 3 | FOLSE, A'Layia | Tri City Flyers | 27.57 | |
| 4 | MORRIS, Kynnedi | Speed Dynamics | 26.72 | |
| 5 | KIMBLE, Rilee | U.S. Express Track Club | 27.37 | |
| 6 | DILLON, Aumni | Hattiesburg Flyers Track Club | 27.83 | |
| 7 | SMITH, Dizaya | KY Track Club | 28.18 | |
| 8 | AYO, Payton | South Baton Rouge Jaguars Trac | 30.14 |