| PL | Athlete | Team | Time | HPL |
|---|---|---|---|---|
| 1 | KIMBLE, Rilee | U.S. Express Track Club | 27.23 | 3 (1) |
| 2 | SMITH, Dizaya | KY Track Club | 27.73 | 3 (2) |
| 3 | DILLON, Aumni | Hattiesburg Flyers Track Club | 27.95 | 3 (3) |
| 4 | FOLSE, A'Layia | Tri City Flyers | 28.33 | 3 (4) |
| 5 | KENNEDY, Aubree | Tri City Flyers | 29.04 | 1 (1) |
| 6 | HARRIS, Briana | Westbank Track Stars | 29.72 | 3 (5) |
| 7 | AYO, Payton | South Baton Rouge Jaguars Trac | 30.02 | 3 (6) |
| 8 | LEWIS, Addison | U.S. Express Track Club | 30.64 | 2 (1) |
| 9 | ADDISON, Tyler | Unattached | 31.77 | 1 (2) |
| 10 | CHARLES, Briley | UDP Track Club | 31.81 | 2 (2) |
| 11 | JOHNSON, Persia | KY Track Club | 32.12 | 2 (3) |
| 12 | AKAMOHEARTEL, Arayah | U.S. Express Track Club | 33.54 | 2 (4) |
| 13 | OLIVER, Khole | Unattached | 33.69 | 1 (3) |
| 14 | MADDING, Makenzye | Metropolitan Track Club | 34.04 | 2 (5) |
| 15 | CRYER, Amanii | Trojan Elite Track | 35.32 | 2 (6) |