| PL | # | Athlete | Team | Time | HPL |
|---|---|---|---|---|---|
| 1 | 16 | CHARLES, Brooklyn | SBR Jags Track and Field | 37.04 | 2 (1) |
| 2 | 38 | RUSSELL, Aniyah | Zachary Wings Elite | 39.33 | 1 (1) |
| 3 | 3 | HARRIS, Melyni | KY Track Club | 39.98 | 2 (2) |
| 4 | 18 | HARRIS, Ty'Riell | SBR Jags Track and Field | 40.95 | 2 (3) |
| 5 | 40 | THOMAS, Aminah | Zachary Wings Elite | 41.16 | 2 (4) |
| 6 | 37 | MCCALEB, Laycee | Zachary Wings Elite | 41.20 | 2 (5) |
| 7 | 36 | MANNING, Trista | Zachary Wings Elite | 41.50 | 2 (6) |
| 8 | 64 | RICHARD, Chole | Motivated Motion Track Team | 41.74 | 1 (2) |
| 9 | 49 | PARKER, Jacee | Endeavor | 48.43 | 1 (3) |