| PL | # | Athlete | Team | Time | HPL |
|---|---|---|---|---|---|
| 1 | 146 | WRIGHT, Amia | Mississippi RoadRunners | 25.07 | 1 (1) |
| 2 | 439 | CIN'CLAIRE, Diamond | Trojan Elite | 25.79 | 2 (1) |
| 3 | 105 | LUCAS, Jaya | Houma Jets | 26.28 | 2 (2) |
| 4 | 443 | GRIFFIN, Dynastee | Trojan Elite | 27.34 | 2 (3) |
| 5 | 91 | COSKY, Mariam | Tri City Flyers | 28.64 | 1 (2) |
| 446 | HOLMES, Myâjha | Trojan Elite | NT |