| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ST JOHN, Jarret | GLADSTONE | 55.32 |
| 2 | STRONG, Preston | KINGSFORD | 53.64 |
| 3 | YESNEY, Colton | NEGAUNEE | 53.48 |
| 4 | SAYEN, Clayton | HOUGHTON | 51.34 |
| 5 | BURKE, Ethan | ISHPEMING WESTWOOD | 52.99 |
| 6 | RUDDEN, Garrett | MARQUETTE | 53.53 |
| 7 | MALHERBE, Julien | MARQUETTE | 54.11 |
| 8 | GUIDEBECK, Kobie | MARQUETTE | 56.51 |