| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | GLADSTONE | A | Robinson, Megan | Marenger, Alexis | Brace, Kloe | Barron, Joyce | 1:03.57 |
| 3 | MARQUETTE | A | Kroll, Abby | Waite, Spenser | King, Julia | Kilpela, Mollie | 53.80 |
| 4 | ESCANABA | A | Farrell, Meghan | Lord, Kaitlyn | Rabitoy, Peyton | Boyle, Courtney | 56.00 |