| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | MARQUETTE | A | Sall, Delaney | Wuorinen, Guinn | Kilpela, Mollie | Puskala, Kaleah | 4:30.00 |
| 2 | GLADSTONE | A | Steineke, Jolan | Sanville, Makayla | Tembreull, Lydia | Larson, Ahna | 4:41.68 |
| 3 | ESCANABA | A | Lord, Kaitlyn | Smith, Anna | Baker, Brooke | Boyle, Courtney | 5:04.36 |