| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Wakefield-Marenisco/Bessemer | A | Libertoski, Adam | Libertoski, Ethan | Imhoff, Leo | Makela, Hayden | NT |
| 2 | Dollar Bay | A | Miller, Ryan | LeClaire, Conner | Norland, Johnny | Benson, Chase | 49.48 |
| 3 | Lake Linden-Hubbell | A | Klein, Kellen | Chopp, Ethan | Klein, Caleb | Crouch, Carter | 47.00 |
| 4 | Bessemer | A | Aspinwall, Andy | Baross, Zach | Braspenick, Alex | Broemer, Collin | 48.37 |
| 5 | Baraga | A | Tangen, Matthew | Funke, Devan | Koskinen, Carter | Rasanen, Presley | NT |
| 6 | Ewen Trout Creek | A | Nordine, Eli | Borseth, Jaden | Doverspike, Julian | Schutz, Colton | NT |