| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | COLONY | B | Little | Dinkle | Pomrenke | Brown | NT | 
| 2 | NOME-BELTZ | A | Ahmasuk | Erickson | Erickson | Hoogendorn | NT | 
| 3 | GRACE CHRISTIAN | A | Davis | Wright | Spence | Zingone | 53.62 | 
| 4 | VALDEZ | A | Aldrich | McCumby | Alfaro | Baczuk | 51.88 | 
| 5 | HOUSTON | A | Arterburn | Hansen | Racette | Johnson | 52.86 | 
| 6 | MOUNTAIN CITY CHRISTIAN ACAD | B | Earhart | Smith | Cavazos | DeGraaf | DNS | 
| 7 | WASILLA | B | Zundel | Parker | Simpson | Johnson | NT | 
| 8 | VALDEZ | B | Wadsworth | Passage | Smilie | Petersen | NT | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 | LATHROP | A | Scott | Skinner | Gosier | Jackson | 48.52 | 
| 2 | SU VALLEY | A | Shockey | Kingery | Gossett | Davidson | 47.95 | 
| 3 | TRI-VALLEY | A | Guhrt | Williams | Wahlin | Mayo | DQ | 
| 4 | COLONY | A | Fieser | Raybon | Weiser | Vito | 47.00 | 
| 5 | WASILLA | A | Musgrave | Corneliussen | Boothby | Wadleigh | 47.44 | 
| 6 | MOUNTAIN CITY CHRISTIAN ACAD | A | Edmondson | Pettit | Stanley | Stevens | 47.90 | 
| 7 | PALMER | A | Estepp | Petersen | Ladd | Cummings | 48.36 | 
| 8 | LATHROP | B | Brooks | Charfauros | Lesseig | Lawson | 49.37 |