| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | HOMER | A | Freeman | Parkinson | Carroll | Cannon | 48.80 |
| 3 | WASILLA | A | Monroe | Compton | Quass-Lee | Slats | 47.35 |
| 4 | PALMER | A | Gibson | Pagel | Garner | Watkins | 48.36 |
| 5 | MAT-SU CAREER AND TECHNICAL | A | Jearlos | Johnson | Hershman | Aparezuk | NT |