| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | KODIAK | A | Academia | Baisa | Longrich | Zalewski | 1:01.79 |
| 2 | PALMER | A | Phillips | Hronkin | Clark | Sanchez | 53.25 |
| 3 | SOLDOTNA | A | Sisley | Brown | Sedivy | Hannevold | 52.49 |
| 4 | COLONY | A | Anderson-Berghoff | Mitchell | Burnett | Larsen | 52.58 |
| 5 | WASILLA | A | Frizzelle | Hafen | Compton | Carter | 55.06 |