| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Colony | C | Malcom | Jackson | Iddings | Smith | NT |
| 2 | Wasilla | A | Kalmykova | Patnode | Pederson | Pokryfki-Greggory | DNS |
| 3 | Colony | A | Johnson | Soeder | Riese | Kistler | 54.74 |
| 4 | Palmer | A | Everett | Knolmayer | Marshall | Zola | 57.02 |
| 5 | Colony | B | Boney | Colwell | Knutsen | Rodriguez | DQ |
| 6 | Redington | A | Simmons | Pike | Gale-Bolger | Howlett | DNS |
| 7 | Su Valley | A | Roderick | Wilson | Kehoe | Shockey | NT |