| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Tusculum |
A | Wright | Taylor | Chellah | Green-White | 4:20.00 | |
| 4 | Wofford |
B | Van Der Linde | Johnson | Ridgeway | Spadafora | 4:14.00 | |
| 5 | Alderson Broaddus |
A | James | Knotts | Fort | Bumbry | 4:10.16 | Mountain East Conference Indoor Track & Field Championships |
| 6 | Saint Francis |
A | Alaban-Tafon | Carpenter | Fleck | Stanavich | 4:11.80 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Virginia Union |
A | White | Lugg | Garrison | Barnett | 4:10.00 | |
| 3 | Washington and Lee |
A | Tulenko | Palethorpe | Evans | Scibelli | 4:09.00 | |
| 4 | Gardner-Webb |
A | Reinboth | Austin | Bour | Ivanov | 4:01.00 | |
| 5 | VMI |
A | Hall | Shank | Lavender | Green | 3:47.91 | Southern Conference Outdoor Championships |
| 6 | Wofford |
A | Cutler | McDevitt | Worf | Jefferson | 3:55.10 |