| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pfeiffer |
B | Veal | Marion | Brown | Chapman | DNS | |
| 3 | Southern Virginia |
A | de la Cruz | Landheim | Moore | Nwaneri | DQ | New Captains Classic & Combined Events |
| 4 | Lynchburg |
A | Brown-Flowers | Powell | Wilson | Charecky | 41.64 | ODAC Outdoor Track & Field Championships |
| 5 | Pfeiffer |
A | Campbell | Bryant | Colson | Brown | 42.56 | Atlantic Independent Track & Field Championship |
| 6 | Virginia Tech Running Club | A | Lopez | Haley | Wendel | DeFosse | 42.70 | |
| 7 | Ferrum |
A | Pineiro | Wade | Tarver | Hamman | 46.00 |