| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Ferrum |
A | Beverley | Hairston | Lewis | Yates | 53.60 | Dennis Craddock Coaches Classic |
| 3 | Hollins |
A | Patterson | Carter | Hylton | Webb-Hawkins | 52.00 | |
| 4 | Randolph |
A | Smith | Forcey | Moore | Turner | 50.72 | ODAC Outdoor Track & Field Championships |
| 5 | Meredith |
A | Cunningham | Reyes | Merna | Olufemi-Ajayi | 51.52 | 6th Annual adidas Trojan Challenge |
| 6 | Virginia Tech Running Club | A | Crawford | Donlon | Ophof | Williams | 52.50 |