| * | Myrtle Beach Collegiate Challenge | 45.95 | Kemp, Simkins, Mitchell, Friday | Seton Hall | 3/13/2010 |
| ! | Doug Shaw Stadium | 44.65 | Johnson, Harris, Bandy, Brockington | South Carolina | 3/22/2014 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Husson |
A | Cotoni | Hubbard | Stiles | Freeman | DNS | North Atlantic Conference Outdoor Championships |
| 2 | Clarion |
A | Sharrow | Lodge | Beard | Uncapher | DQ | PSAC Outdoor Track & Field Championships |
| 3 | North Greenville |
A | Wilson | Smiling | Vaughn | Cox | 48.68 | 2022 Conference Carolinas Track & Field Championships |
| 4 | Coastal Carolina |
A | Clemens | Gordon | Kinloch | St. Cyr | DNF | NCAA Division I Outdoor Track & Field Championships |
| 5 | Truett-McConnell |
A | Arnold | Hall | Wilmoth | Nakanwagi | DNF | Appalachian Athletic Conference Track & Field Championships |
| 6 | Allen |
A | Patterson | Moorer | Passmore | Jones | 50.66 | SWU GottaRun Invite |
| 7 | Clarion |
B | Redwood | Snyder | Fenice | Serrano | 50.84 | PSAC Outdoor Track & Field Championships |
| 8 | Alderson Broaddus |
A | Colas | Knotts | Murphy | Alnatour | DNF | Mountain East Conference Track & Field Championships |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Wilmington (Del.) |
A | Brown | Turner | Hunt | Mustapha | DNS | Myrtle Beach Collegiate Challenge |
| 4 | Geneva |
A | Swartzbaugh | Holdeman | Parnell | Shaporka | DNS | Grove City College Mini Meet |
| 5 | A | Redding | Charles | Emelo | Shannon | DQ | North Atlantic Conference Outdoor Championships | |
| 6 | Alfred |
A | Durso | Young | Ryckman | Spila | 54.40 | St. John Fisher Cardinal Classic |