| * | Myrtle Beach Collegiate Challenge | 40.25 | Cue, Williams, Davis, Dukes | LIU Brooklyn | 3/12/2016 |
| ! | Doug Shaw Stadium | 39.91 | Johnson, Young, Tyler, Spreads | Charleston Southern | 4/21/2012 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Alderson Broaddus |
A | Jean | Blunt | Monpas | Napier | DNF | Mountain East Conference Track & Field Championships |
| 2 | Wilmington (Del.) |
A | Fountain | Bailey | Spencer | Levons | DNS | Mount Olive First Chance |
| 3 | North Greenville |
A | Hamby | Lawton | White | Webb | 41.71 | Electric City Invitational |
| 4 | Coastal Carolina |
A | Barlow | Wilkins | Hardy | Harris | DNF | UofSC Open |
| 5 | Truett-McConnell |
A | Liesen | Alamia | Oaks | Wilgus | 40.95 | Appalachian Athletic Conference Track & Field Championships |
| 6 | Allen |
A | Brock | White | Burley | Myers | 42.10 | SWU Susan Rouse Invite |
| 7 | Geneva |
A | Trexler | Blessing | Andrews | Lewis | 42.28 | WVU Stan Romanoski Open |
| 8 | New Haven |
A | Holland | Williams | Colson | Lorent | 42.34 | Jay Carisella Coaches Invitational |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | A | Pelletier | Plamondon | Rounds | Raye | DQ | North Atlantic Conference Outdoor Championships | |
| 4 | Husson |
A | Hart | Ramos | Feeney | Zapata | 43.44 | North Atlantic Conference Outdoor Championships |
| 5 | A | Gray | VeraCruz | Joinville | Sarver | DNF | 2022 NCCAA Outdoor Track & Field Championships | |
| 6 | A | Fofang | McGlothin | Knowles Tener | Marcelin | 44.12 | Bethany Invitational |