| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 135 | ROACH, Jayden | FR | Rio Grande |
2:22.37 | University of Charleston Golden Eagle Invitational |
| 2 | 155 | KERNS, Abrielle | FR | Rio Grande |
2:54.46 | River States Conference Indoor Track & Field Championships |
| 3 | 4 | PARKS, Victoria | FR | Concord |
2:58.27 | High Point - Bob Davidson Memorial |
| 4 | 27 | VAUGHT, Brittany | SR | Concord |
3:26.84 | Marietta Open |
| 5 | 25 | BENTLEY, Baylee | FR | Concord |
NT |