| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Rio Grande |
A | Williams | Hetzler | Hunt | Gries | 45.00 | |
| 3 | Concord |
A | Cochran | Thomas | Gardner | Patterson-Bryant | 43.21 | University of Charleston Golden Eagle Invitational |
| 4 | Concord |
B | Sweeney | Porterfield | Sandy | Turner | 45.00 |