| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 223 | BANKS, Marek | JR | Bluefield |
52.22 | |
| 2 | 229 | BANKS, Mykkel | JR | Bluefield |
51.90 | |
| 3 | 77 | DAVIS, Jeremiah | FR | Averett |
50.83 | Vertklasse |
| 4 | 230 | HEWITT, Kaleb | SO | Bluefield |
51.00 | |
| 5 | 175 | PENNIX, McKinley | FR | 51.96 | Commonwealth College Opener | |
| 6 | 227 | LLORENC, Porquer | JR | Bluefield |
53.00 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 158 | MARTINEZ, Ivan | JR | WVU Tech |
55.61 | Montreat College Open |
| 3 | 137 | WILSON, Zach | SO | Concord |
54.00 | |
| 4 | 180 | JONES, Jalen | FR | 54.83 | HBCU Classic | |
| 5 | 93 | BROWN, Christopher | FR | Averett |
58.70 | Vertklasse |
| 6 | 80 | ALIMA-GRIMSHAW, Kevin | FR | Averett |
1:00.53 | Vertklasse |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 73 | GRAYTON, James | FR | Concord Unattached | 59.00 | |
| 3 | 28 | STANTON, Blake | SO | Charleston (W.V.) |
NT |