| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | James River (Buchanan) | A | Binns | Firebaugh | Orent | VanBuren | 55.11 | Verified |
| 3 | Carroll County | A | 54.31 | Verified | ||||
| 4 | Glenvar High School | A | 52.77 | Verified | ||||
| 5 | Hidden Valley High School | A | Lee | Thomson | Guerrero | Atwood | 53.13 | Verified |
| 6 | Auburn | A | Gillenwater | Pack | Rorrer | Lewis | 54.50 | Verified |
| 7 | Giles | A | Ellison | Sherman | Taylor | Lane | 57.64 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Christiansburg | A | Moles | Logwood | Bishop | Haley | 52.50 | Verified |
| 2 | Abingdon High School | A | Blackmon | Cvetkovski | Odum | Hopson | 51.56 | Verified |
| 3 | Heritage (Lynchburg) | A | Hayden | Davis | Tune | Porter | 51.25 | Unverified: The Penn Relays Carnival Present |
| 4 | Blacksburg High School | A | 49.75 | Verified | ||||
| 5 | Salem (Roanoke) | A | 50.64 | Verified | ||||
| 6 | Pulaski County | A | Lyman | Secrist | Viers | Jackson | 51.47 | Verified |
| 7 | Cave Spring High School | A | Cooper | Hertzberg | Mather | Cavicchio | 51.60 | Verified |
| 8 | Floyd County | A | Ballinger | Brewer | Quesenberry | Spangler | 52.66 | Verified |