| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Woodrow Wilson Middle School | A | Smith | Alexander | Early | Matheney | NT | Unverified: |
| 3 | Floyd County | A | Bakely | Cox | Fletcher | Keith | 56.11 | Unverified: |
| 4 | John P. Fishwick Middle School | A | Coleman | Ragland | Lee-Torrez | Preston | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lucy Addison Middle School | A | Guerrant | Gonzalez | Brown | Haynes | 54.53 | Verified |
| 3 | Blacksburg Middle School | A | Keys | Liu | Jones | Daily | DNF | Unverified: |
| 4 | Christiansburg Middle School | A | Scaggs | Tilley | Gaston | Cotto | 53.10 | Unverified: |
| 5 | Clifton Middle School | A | Godfrey | Godfrey | Nicely | Christian | DQ | Unverified: |