| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Columbia (S.C.) |
A | Bailey | Roach | Linen | Reinsel | DNF | Appalachian Athletic Conference Track & Field Championships |
| 2 | Lincoln (Pa.) |
A | Hall | Amaker | Childs | Butumbi | 3:26.34 | |
| 3 | Lenoir-Rhyne |
B | White | Heath | McGill | Owens | DNS | |
| 4 | Roanoke |
A | Crawford | Heffinger | Fowler | Camobreco | DNS | ODAC Outdoor Track & Field Championships |
| 5 | Lenoir-Rhyne |
A | Besong | Sisson | Matau | Crumpler | 3:10.27 | Charlotte Invitational |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Livingstone |
B | Mungo | Nunn | Fryson | Axon | 3:30.00 | |
| 2 | Concord |
A | Gardner | Bhebhe | Jackson-Darby | Kwakye | 3:30.00 | |
| 3 | Lincoln (Pa.) |
B | Marable | Rodriguez | Scott | Baker | 3:29.56 | |
| 4 | Virginia Union |
A | Laws | Phifer | Hinton | Ssengo | 3:29.37 | George Mason Father Diamond Invitational 2020 |
| 5 | Columbia (S.C.) |
B | Avant | Mitchell | Peeples | Thompson | 3:27.01 | Appalachian Athletic Conference Track & Field Championships |
| 6 | Columbia (S.C.) |
C | Wright | Greene | Scott | DNF | Appalachian Athletic Conference Track & Field Championships |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Roanoke |
B | Estes | Hamman | Graham | Morin | 3:33.00 | |
| 2 | Randolph |
A | Lambert | Kurtz | Thompson | Brown | 3:35.00 | |
| 3 | Livingstone |
C | Freeman | Jenkins | Ridenhour | Steele | 3:35.00 | |
| 4 | Emory & Henry |
A | Yeboah | Pecina | Pecina | Thompson | 3:34.24 | ODAC Indoor Track & Field Championships |
| 5 | Carson-Newman |
A | Blaylock | Bostick | Dupler | Laribo | 3:30.44 | |
| 6 | Carson-Newman |
B | Blackmon | Charamba | Jones | Lemao | 3:31.33 | |
| 7 | Livingstone |
A | Bradford | Wagner | Gilbert | Strader | 3:21.54 | VMI Winter Relays |